• not heat fixed properly, must be heated to steaming for 5 minutes, must be kept wet during steaming with malichite green
  • no actually the cell is not susposted to be green. Since malachite green is water-soluble and does not adhere well to the cell, and since the vegetative cells have been disrupted by heat, the malachite green rinses easily from the vegetative cells, allowing them to readily take up the counterstain.

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